Lionheart Health Files Patent for Bioelectric-Enhanced Klotho Nanoflowers Designed for Aging Reversal and Healthspan

Lionheart Health, Inc., a leader in bioelectric regeneration and longevity biotechnology, today announced the filing of a groundbreaking non-provisional U.S. patent covering its new platform technology: Bioelectric-Enhanced Klotho Nanoflowers for Aging Reversal and Healthspan Extension.

The newly filed patent protects a transformative therapeutic system that combines nanoflower-format nanoparticles, bioelectric signaling sequences, and Klotho-modulating biologics to target the cellular and mitochondrial drivers of aging. The invention integrates several proprietary Lionheart technologies, including BodStim™, BrainBand™, HairCell™, ErectiStim TM, Second Brain TM, InStim TM, EyeCell TM, EarCell TM, BioLeonhardt TM, KidneyCell TM, LiverCell TM, RegenaLung TM, Pantropelstin creaCell TM, TestiStim™, ErectiStim™, and advanced Klotho-based peptides and gene-expression tools.

A Novel Approach to Reversing Cellular Aging

The patented platform centers on engineered “nanoflowers”—flower-shaped hybrid nanoparticles capable of:

• Upregulating Klotho, a longevity-associated protein shown to improve brain, kidney, cardiovascular, and metabolic health
• Restoring mitochondrial function by enhancing ATP production and reducing oxidative stress
• Delivering targeted bioactive agents including peptides, plasmids, mRNA, and CRISPRa activators
• Acting as nanozyme catalysts that reduce ROS and improve cellular resilience
• Enhancing regenerative signaling when activated by precise bioelectric patterns

The nanoflowers are engineered to respond to Lionheart’s proprietary bioelectric stimulation sequences, which increase cellular uptake, activate intracellular pathways, and amplify biological performance.

This invention represents the convergence of three of the most powerful emerging technologies in biotechnology—bioelectric signaling, nanomedicine, and Klotho modulation,” said Howard J. Leonhardt, Founder, Lead Inventor and Chief Executive Officer of Lionheart Health. “Our goal is to create a system that reverses biological age at the cellular level and enhances healthspan safely, non-invasively, and affordably.

Nanoflowers & Bioelectric Stimulation Are Synergistic

The nanoflowers are specifically engineered to respond to bioelectric fields.
The bioelectric signals increase:

• cellular uptake of nanoflowers,

• intracellular transport, and

• activation of the nanoflower’s catalytic and genetic payloads.

Thus, nanoflowers require bioelectric activation for optimal performance, and the bioelectric component is designed to operate in the presence of the nanoflowers.

Bioelectric stimulation is applied to:

• open ion channels,

• improve endocytosis,

• direct nanoflowers toward mitochondria, and

• trigger therapeutic payload release.

Klotho Upregulation Is a Combined Output of Both Components

Klotho expression increases due to:

• nanoflower-delivered genetic or peptide payloads, and

• bioelectric activation of promoter and epigenetic pathways.

Thus, neither nanoflowers nor bioelectric stimulation alone can produce the full Klotho-enhancing effect, demonstrating a combined therapeutic benefit mechanism.

Designed for:

SYSTEM-WIDE HEALTHSPAN IMPROVEMENT

Mitochondrial Biogenesis

Activation of PGC-1α, NRF1/2, TFAM, leading to new mitochondrial formation.

Reduction of Cellular Senescence

Lower oxidative stress reduces p16/p21 pathways and senescence-associated β-gal.

Stabilization of Biological Age

Combined mitochondrial repair + Klotho enhancement reduces multi-system aging markers.

Inspired by the Future of Regenerative Medicine

Klotho is widely recognized as one of the most promising longevity molecules ever discovered. Decreased Klotho levels are associated with accelerated aging, cognitive decline, inflammation, vascular calcification, kidney dysfunction, and frailty. Increasing Klotho expression has demonstrated benefits in preclinical models of brain aging, muscle repair, cardiac healing, kidney protection, and metabolic health.

Lionheart’s new platform advances this science by enabling targeted Klotho delivery and activation inside mitochondrial and cellular compartments that are most responsible for aging.

“Bioelectric-enhanced Klotho nanoflowers give us a programmable, multi-modal tool to address the root causes of aging—declining energy production, rising inflammation, and loss of regenerative capacity,” said Dr. Leslie Miller, Chief Medical Officer and former President of the International Society for Heart and Lung Transplantation. “This filing marks a major milestone in longevity therapeutics.”

Positioning Lionheart as a Global Leader in Healthspan Technologies

The patent filing adds to Lionheart Health’s growing intellectual property portfolio spanning:

• Bioelectric-controlled protein expression including klotho, sestrins, sirtuins, NANOG, apelin, SDF1, PDGF, LIM, S100a, Tropelastin, COL17A1, VEGF and more.
• Stem-cell mobilization and tissue regeneration
• Mitochondrial restoration technologies
• Klotho-based gene therapy and peptide therapeutics
• Regenerative aesthetic and wellness platforms

OPTIONAL COMPONENTS include:

Closed-Loop Adaptive Control

Sensors measure mitochondrial markers or electrophysiological signals to adapt stimulation patterns.

Multi-Modal Integration

Nanoflowers + bioelectric + peptides + red light + PEMF + regenerative support including cell and gene therapy.

Repeat Cycle Integration

Therapy will be delivered in studies in iterative sessions (baseline, 3-month, 6-month, 12-month intervals).

Lionheart Health is currently preparing for expanded clinical evaluation within its network of Lionheart Longevity & Wellness Licensed Clinics across the U.S. and outside of the USA. Company plans to integrate bioelectric-enhanced Klotho nanoflowers into its XPRIZE Healthspan competition program.

About Lionheart Health, Inc.

Lionheart Health, Inc. is pioneering a new era of bioelectric, regenerative, and longevity technologies. The company develops clinically validated systems for organ repair, cellular rejuvenation, hair regeneration, muscle restoration, metabolic optimization, and age reversal. Its mission is to extend healthspan and improve quality of life for people worldwide through safe, non-invasive, high-impact technologies.

Learn more at:

www.LionheartHealthStim.com

www.LionheartLongevity.com

www.LeonhardtVentures.com

Media Contact:

Lionheart Health Communications & Customer Service

Email: [email protected]